In this work, the double cantilever beam (DCB) test is analysed in order to evaluate the combined effect of temperature and moisture on the mode I fracture toughness of adhesives used in the automotive industry. Very few studies focus on the combined effect of temperature and moisture on the mechanical behaviour of adhesive joints. To the authors' knowledge, the simultaneous effect of these conditions on the fracture toughness of adhesive joints has never been determined. Specimens using two different adhesives for the automotive industry were subjected to two different ageing environments (immersion in distilled water and under 75% of relative humidity). Once they were fully degraded, they were tested at three different temperatures (-40, 23 and 80 degrees C), which covers the range of temperature an adhesive for the automotive industry is required to withstand. The aim is to improve the long term mechanical behaviour prediction of adhesive joints. The DCB substrates were made of a high strength aluminium alloy to avoid plastic deformation during test. The substrates received a phosphoric acid anodisation to improve their long term adhesion to the adhesive. Results show that even though a phosphoric acid anodization was applied to the adherends, when the aged specimens were tested at room temperature and at 80 degrees C, they suffered interfacial rupture. At -40 degrees C, however, cohesive rupture was observed and the fracture toughness of the aged specimens was higher.