Dynamic nuclear polarization (DNP) via the dissolution method has alleviated the insensitivity problem in liquid-state nuclear magnetic resonance (NMR) spectroscopy by amplifying the signals by several thousand-fold. This NMR signal amplification process emanates from the microwave-mediated transfer of high electron spin alignment to the nuclear spins at high magnetic field and cryogenic temperature. Since the interplay between the electrons and nuclei is crucial, the chemical composition of a DNP sample such as the type of free radical used, glassing solvents, or the nature of the target nuclei can significantly affect the NMR signal enhancement levels that can be attained with DNP. Herein, we have investigated the influence of C-13 isotopic labeling location on the DNP of a model C-13 compound, sodium acetate, at 3.35 T and 1.4 K using the narrow electron spin resonance (ESR) line width free radical trityl OX063. Our results show that the carboxyl C-13 spins yielded about twice the polarization produced in methyl C-13 spins. Deuteration of the methyl C-13 group, while proven beneficial in the liquid-state, did not produce an improvement in the C-13 polarization level at cryogenic conditions. In fact, a slight reduction of the solid-state C-13 polarization was observed when 2H spins are present in the methyl group. Furthermore, our data reveal that there is a close correlation between the solid-state C-13 T-1 relaxation times of these samples and the relative C-13 polarization levels. The overall results suggest the achievable solid-state polarization of C-13 acetate is directly affected by the location of the C-13 isotopic labeling via the possible interplay of nuclear relaxation leakage factor and cross-talks between nuclear Zeeman reservoirs in DNP.