A simple voltammetric sensor has been developed for direct determination of important biomarker uric acid (UA) based on poly bromophenol blue-gold nanoparticles composite modified glassy carbon electrode. Scanning electron microscopy, atomic force microscopy and various electrochemical techniques were used for characterization of modified electrode surface. The electrooxidation of UA in 0.1 M NaOH was excellently catalyzed by modified electrode. The anodic peak corresponding to the oxidation of UA was observed at a potential of -105 mV by Square wave voltammetry (SWV). The oxidation peak current was increased linearly on increasing the concentration of UA in the range 1.0 x 10(-3) to 2.0 x 10(-5) M (R-2 = 0.994) with a limit of detection 2.2 x 10(-6) M. The proposed method was effectively applied to determine Uric acid in artificial blood serum and urine samples. The developed sensor would be a potential alternative tool for UA determination in biological samples. (C) 2017 The Electrochemical Society. All rights reserved.