Phosphoinositide 3-kinase gamma (PI3K gamma) is a lipid kinase that plays a crucial role in cell migration, chemotaxis, oxidative burst and myocardial contractility. It is activated downstream of G protein-coupled receptors (GPCRs) and small GTPases of Ras superfamily. PI3K gamma is a heterodimer composed of a catalytic and a regulatory subunit that is expressed mostly in hematopoietic cells and in the heart. Although it has attracted a lot of attention because of its link with tumor inflammation and heart diseases, its regulation is still not fully understood. This can be attributed to the absence of high-resolution structural details of the PI3K gamma heterodimer. Here we describe the design and purification of PI31

Keywords:Phosphoinositide 3-kinas;Pl3K;Signaling;Purification;Baculovirus;Sf9;HDX-MS;Crystallization