화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Journal of Industrial and Engineering Chemistry, Vol.53, 360-370, September, 2017
DOI10.1016/j.jiec.2017.05.007  Export Citation
Preparation and evaluation of visible-light cured glycol chitosan hydrogel dressing containing dual growth factors for accelerated wound healing
Dae Hyeok Yang, Dong In Seo, Deok-Won Lee1, Suk Ho Bhang2, Kyeongsoon Park3, Goo Jang4, Chun Ho Kim5, and Heung Jae Chun†
  • Institute of Cell and Tissue Engineering, Department of Biomedical Sciences, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea
  • 1Department of Oral and Maxillofacial Surgery, Kyung Hee University Dental Hospital at Gandong, Kyung Hee University, Seoul 05278, Republic of Korea
  • 2School of Chemical Engineering, Sungkyunkwan University, Suwon 16419, Republic of Korea
  • 3Department of Systems Biotechnology, Chung-Ang University, Anseong 17546, Republic of Korea
  • 4Laboratory of Theriogenology and Biotechnology, Department of Veterinary Clinical Science, College of Veterinary Medicine and the Research Institute of Veterinary Science, Seoul National University, Seoul 08826, Republic of Korea
  • 5Laboratory of Tissue Engineering, Korea Institute of Radiological and Medical Science, Seoul 139240, Republic of Korea
E-mail:
The visible light-cured glycol chitosan (GC) hydrogel systems for the sustained release of growth factors (GFs) were prepared, and their efficacies in wound healing acceleration were investigated. Vascular endothelial growth factor (VEGF) and platelet-derived growth factor-BB (PDGF-BB) were selected as the dual GFs and commercially available Duoderm1 was used as control. In order to increase intermolecular chain mobility, methoxy (polyethylene glycol) acetic acid (MPEG-COOH) was conjugated to GC through condensation reaction resulting in MPEG grafted GC (MPEG-g-GC). As a crosslinker, glycidyl methacrylate was covalently conjugated to the amine group of MPEG-g-GC (MPEG/GM-g-GC), and crosslinking of MPEG/GM-g-GC chains were made by visible light irradiation (MPEG-g-GC hydrogels). To enhance wound healing efficacy, specific amounts of GFs was incorporated into the crosslinking solutions at this photo-curing stage (VEGF/MPEG-g-GC, PDGF/MPEG-g-GC and VEGF/PDGF/MPEG-g-GC). The grafting of MPEG plasticized the samples yielding low viscoelastic properties, ranging from 56 Pa to 58 Pa. In vitro release test showed that GFs were rapidly released within 24 h, and released in a sustained manner thereafter. In vivo studies showed that the GF-loaded samples further enhanced wound healing compared to control. Particularly, VEGF/PDGF/MPEG-g-GC, dual GFs releasing hydrogel, showed outstanding granulation effects among samples.
Keywords:Visible light;Glycol chitosan (GC) hydrogel;Vascular endothelial growth factor (VEGF);Platelet-derived growth factor-BB (PDGFBB);Wound healing
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