Preparation and anticancer effect of transferrin-modified pH-sensitive polymeric drug nanoparticle for targeted cancer therapy

Tae-Hun Kim; Gyeong-Won Jeong; Jae-Woon Nah

Journal of Industrial and Engineering Chemistry, Vol.54, 298-303, October, 2017

DOI10.1016/j.jiec.2017.06.004 Export Citation

Preparation and anticancer effect of transferrin-modified pH-sensitive polymeric drug nanoparticle for targeted cancer therapy

Tae-Hun Kim, Gyeong-Won Jeong¹, and Jae-Woon Nah^{1, †}

BioALPHA Co., Ltd, Sengnam-si 22323, Republic of Korea
¹Department of Polymer Science and Engineering, Sunchon National University, Jeonnam 57922, Republic of Korea

E-mail:

The doxorubicin-conjugated O-carboxymethyl chitosan (OCAD) was synthesized by hydrazone bond and transferrin was introduced to OCAD for targeted cancer therapy. The OCAD was analyzed by 1H NMR and, diameter of OCAD and TPOCAD were confirmed 230 nm and 250 nm by DLS, respectively. The release profile of OCAD showed that acidic pH was rapidly released than neutral pH. To compare cellular uptake of OCAD and TPOCAD, its internalization was analyzed by CLSM. In addition, cytotoxicity of TPOCAD was accomplished by MTT assay. Therefore, TPOCAD-polymeric drug has a potential ability as drug delivery system for effective chemotherapy against cancer cells.

Keywords:Polymeric drug;pH-sensitive;O-Carboxymethyl chitosan;Transferrin;Doxorubicin

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[Referenced By]

[1] Liu L, Zhao YF, Han WH, Chen T, Hou GH, Tong XZ, Asian Pac. J. Trop. Med., 9, 1101 (2016)

[2] Farhane Z, Bonnier F, Byrne HJ, Anal. Bioanal. Chem., 409, 1333 (2017)

[3] Barooah N, Kunwar A, Khurana R, Bhasikuttan AC, J. Mohanty, Chem. Asian J., 12, 122 (2017)

[4] Huang SC, Wu JF, Saovieng S, Chien WH, Hsu MF, Li XF, Lee SD, Huang CY, Huang CY, Kuo CH, J. Cachexia Sarcopenia Muscle, 8, 277 (2017)

[5] Zhu D, Tao W, Zhang H, Liu G, Wang T, Zhang L, Zeng X, Mei L, Acta Biomater., 30, 144 (2016)

[6] Raju KR, Ambhore NS, Mulukutla S, Gupta S, Murthy V, Kumar MN, Madhunapantula SR, Kuppuswamy G, Elango K, Med. Hypotheses, 87, 75 (2016)

[7] Masood F, Mater. Sci. Eng. C-Biomimetic Supramol. Syst., 60, 569 (2016)

[8] Xiong L, Luo Q, Wang Y, Li X, Shen Z, Zhu W, Chem. Commun., 51, 14644 (2015)

[9] Troiano G, Nolan J, Parsons D, Van Geen Hoven C, AAPS J., 18, 1354 (2016)

[10] Beck-Broichsitter M, Nicolas J, Couvreur P, Eur. J. Pharm. Biopharm., 97, 304 (2015)

[11] Jones MC, Ranger M, Leroux JC, Bioconjug. Chem., 14, 774 (2003)

[12] Chen D, Wang H, J. Nanosci. Nanotechnol., 14, 983 (2014)

[13] Lim EK, Chung BH, Chung SJ, Current Drug Targets, (2016).

[14] Zhang W, Dong D, Li P, Wang D, Mu H, Niu H, Duan J, Carbohydr. Polym., 139, 75 (2016)

[15] Cao J, Su T, Zhang L, Liu R, Wang G, He B, Gu Z, Int. J. Pharm., 471, 28 (2014)

[16] Nie SY, Lin WJ, Yao N, Guo XD, Zhang LJ, ACS Appl. Mater. Interfaces, 6, 17668 (2014)

[17] Pan S, Mo J, Zheng H, Yi W, Huang D, Wang B, Sheng Wu Yi Xue Gong Cheng Xue Za Zhi., 26, 347-359 (2009).

[18] Zhu A, Dai S, Li L, Zhao F, Colloids Surf. B: Biointerfaces, 47, 20 (2006)

[19] Demarchi CA, Debrassi A, Fde CB, Correa R, Filho VC, Rodrigues CA, Nedelko N, Demchenko P, Slawska-Waniewska A, Dluzewski P, Greneche JM, Soft Matter, 10, 3441 (2014)

[20] Thanou M, Henderson S, Kydonieus A, Elson C, J. Control. Release, 117, 171 (2007)

[21] Zhu AP, Zhao F, Fang N, J. Biomed. Mater. Res., 86, 467 (2008)

[22] Tortorella S, Karagiannis TC, J. Membr. Sci., 247, 291 (2014)

[23] Li S, Amat D, Peng Z, Vanni S, Raskin S, De Angulo G, Othman AM, Graham RM, Leblanc RM, Nanoscale, 8, 16662 (2016)

[24] Jeong GW, Park SC, Choi C, Nam JP, Kim TH, Choi SK, Park JK, Nah JW, Int. J. Pharm., 488, 165 (2015)

[25] Trowbridge IS, Prog. Allergy, 45, 121 (1988)

[26] Nam JP, Kim DG, Kim YB, Jeong YI, Jang MK, Nah JW, J. Chitin Chitosan, 13, 105 (2008)

[27] Wilke A, Hesse H, Gorg C, Maisch B, Oncology, 56, 110 (1999)

[28] Na K, Korean J. Gastroenterol., 49, 314 (2007)

[29] Hayashi T, Yao S, Crain B, Promessi VJ, Shyu L, Sheng C, Kang M, Cottam HB, Carson DA, Corr M, PLoS One, 10, e01298 (2015)

[30] Daniels TR, Bernabeu E, Rodriguez JA, Patel S, Kozman M, Chiappetta DA, Holler E, Ljubimova JY, Helguera G, Penicheta ML, Biochim. Biophys. Acta, 1820, 291 (2012)

[31] Okazaki F, Matsunaga N, Okazaki H, Utoguchi N, Suzuki R, Maruyama K, Koyanagi S, Ohdo S, Cancer Res., 70, 6238 (2010)

[32] Willingham MC, Pastan I, J. Histochem. Cytochem., 33, 59 (1985)