Enhancement of T2-weighted MR contrast using heparin for cell tracking in vivo

Yong Hwa Hwang; Min Jin Jeong; Min Jun Kim; Jeong Kon Kim; Dong Yun Lee

Journal of Industrial and Engineering Chemistry, Vol.55, 183-190, November, 2017

DOI10.1016/j.jiec.2017.06.043 Export Citation

Enhancement of T2-weighted MR contrast using heparin for cell tracking in vivo

Yong Hwa Hwang, Min Jin Jeong, Min Jun Kim, Jeong Kon Kim¹, and Dong Yun Lee^†

Departments of Bioengineering, College of Engineering, and BK21 PLUS Future Biopharmaceutical Human Resources Training and Research Team, and Institute of Nano Science & Technology (INST), Hanyang University, Seoul 04763, Republic of Korea
¹Department of Radiology, Asan Medical Center, University of Ulsan, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Republic of Korea

E-mail:

Superparamagnetic iron oxide (SPIO) nanoparticle has emerged as a novel probe for noninvasively cellular tracking using magnetic resonance imaging. Here we developed SPIO nanoparticles coated with biocompatible heparins, i.e., low-molecular-weight-heparin (LMWH) or unfractionated heparin (UFH). These heparin-SPIO nanoparticles showed good crystallizability and stability without self-aggregation for 50 days. Also, UFH-SPIO nanoparticle had lower relaxation time with higher relaxivity. Moreover, they were efficiently internalized into cells without cytotoxicity and had faster saturated magnetization. Furthermore, the implanted SPIO nanoparticles-labeled Ins-1 cells were strongly visualized in vivo MR imaging. Collectively, heparin-based SPIO nanoparticles could have a great potential for in vivo cellular tracking.

Keywords:MRI;Superparamagnetic iron oxide (SPIO);Heparin;Cellular tracking;Cell transplantation

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[2] Thakur ML, Lavender JP, Arnot RN, Silvester DJ, Segal AW, J. Nucl. Med., 18, 1014 (1977)

[3] Ahrens ET, Bulte JWM, Nat. Rev. Immunol., 13, 755 (2013)

[4] Shapiro EM, Skrtic S, Sharer K, Hill JM, Dunbar CE, Koretsky AP, Proc. Natl. Acad. Sci. U. S. A., 101, 10901 (2004)

[5] Toso C, Zaidi H, Morel P, Armanet M, Andres A, Pernin N, Baertschiger R, Slosman D, Buhler LH, Bosco D, Berney T, Transplantation, 79, 353 (2005)

[6] Bulte JW, Kraitchman DL, NMR Biomed., 17, 484 (2004)

[7] Guenoun J, Ruggiero A, Doeswijk G, Janssens RC, Koning GA, Kotek G, Krestin GP, Bernsen MR, Contrast Media Mol. Imaging, 8, 165 (2013)

[8] Aoki I, Takahashi Y, Chuang KH, Silva AC, Igarashi T, Tanaka C, Childs RW, Koretsky AP, NMR Biomed., 19, 50 (2006)

[9] McCarthy JR, Weissleder R, Adv. Drug Deliv. Rev., 60, 1241 (2008)

[10] Salgueirino-Maceira V, Correa-Duarte MA, Adv. Mater., 19(23), 4131 (2007)

[11] Gupta AK, Naregalkar RR, Vaidya VD, Gupta M, Nanomedicine, 2, 23 (2007)

[12] Hawrylak N, Ghosh P, Broadus J, Schlueter C, Greenough WT, Lauterbur PC, Exp. Neurol., 121, 181 (1993)

[13] Yang CY, Tai MF, Chen ST, Wang YT, Chen YF, Hsiao JK, Wang JL, Liu HM, J. Appl. Phys., 105 (2009)

[14] Ke YQ, Hu CC, Jiang XD, Yang ZJ, Zhang HW, Ji HM, Zhou LY, Cai YQ, Qin LS, Xu RX, J. Neurosci. Methods, 179, 45 (2009)

[15] Zhu WZ, Li X, Qi JP, Tang ZP, Wang W, Wei L, Lei H, Biomed. Environ. Sci., 21, 420 (2008)

[16] Suzuki Y, Zhang S, Kundu P, Yeung AC, Robbins RC, Yang PC, Magn. Reson. Med., 57, 1173 (2007)

[17] Park BH, Jung JC, Lee GH, Kim TJ, Lee YJ, Kim JY, Kim YW, Jeong JH, Chang YM, Colloids Surf. A: Physicochem. Eng. Asp., 313, 145 (2008)

[18] Arbab AS, Yocum GT, Wilson LB, Parwana A, Jordan EK, Kalish H, Frank JA, Mol. Imaging, 3, 24 (2004)

[19] Berman SMC, Walczak P, Bulte JWM, WIREs Nanomed. Nanobiotechnol., 3, 343 (2011)

[20] Jung MJ, Lee SS, Hwang YH, Jung HS, Hwang JW, Kim MJ, Yoon S, Lee DY, Biomaterials, 32, 9391 (2011)

[21] Lee DY, Macromol. Res., 19(8), 843 (2011)

[22] Dobrovolskaia MA, Aggarwal P, Hall JB, McNeil SE, Mol. Pharm., 5, 487 (2008)

[23] Chouly C, Pouliquen D, Lucet I, Jeune JJ, Jallet P, J. Microencapsul., 13(3), 245 (1996)

[24] Decuzzi P, Ferrari M, Biomaterials, 27, 5307 (2006)

[25] Decuzzi P, Lee S, Bhushan B, Ferrari M, Ann. Biomed. Eng., 33, 179 (2005)

[26] Decuzzi P, Causa F, Ferrari M, Netti PA, Ann. Biomed. Eng., 34, 633 (2006)

[27] Chavanpatil MD, Khdair A, Panyam J, J. Nanosci. Nanotechnol., 6, 2651 (2006)

[28] Linhardt RJ, Gunay NS, Semin. Thromb. Hemost., 25, 5 (1999)

[29] Alban S, Handb. Exp. Pharmacol. 211 (2012).

[30] Arepally GM, Ortel TL, Annu. Rev. Med., 61, 77 (2010)

[31] Le Templier G, Rodger MA, Curr. Opin. Pulm. Med., 14, 403 (2008)

[32] Lee DY, Park K, Kim SK, Park RW, Kwon IC, Kim SY, Byun Y, Clin. Cancer Res., 14, 2841 (2008)

[33] Kim SK, Lee DY, Kim CY, Nam JH, Moon HT, Byun Y, J. Control. Release, 123, 155 (2007)

[34] Lee DY, Lee J, Lee S, Kim SK, Byun Y, J. Control. Release, 123, 39 (2007)

[35] Lee JH, Jung MJ, Hwang YH, Lee YJ, Lee S, Lee DY, Shin H, Biomaterials, 33, 4861 (2012)