Crude glycerol has been widely investigated as a renewable carbon source for biodiesel production. In the present study, this feedstock was supplemented by various inducers: surfactants and oils to enhance lipid and lipase production by the newly isolated yeast, Yarrowia lipolytica. Results have shown that a culture medium composed of olive oil could enhance lipase activity at 25 U/mL and lipid content up to 35% (w/w). The fortification of the medium with crustacean waste increased the lipase activity up to 38 U/mL. The hydrolytic activity of the extracellular lipases produced in mentioned medium was satisfactory and opened avenues for other biotechnological processes. (C) 2017 Elsevier Ltd. All rights reserved.