Resveratrol has long been used as an ingredient in functional foods. Currently, Polygonum cuspidatum extract is the greatest natural source for resveratrol because of high concentrations of glycosidic-linked resveratrol. Thus, developing a cost-effective procedure to hydrolyze glucoside could substantially enhance resveratrol production from P. cuspidatum. This study selected Dekkera bruxellensis from several microorganisms based on its bioconversion and enzyme-specific activities. We demonstrated that the cells could be reused at least nine times while maintaining an average of 180.67 U/L beta-glucosidase activity. The average resveratrol bioconversion efficiency within five rounds of repeated usage was 108.77 +/- 0.88%. This process worked effectively when the volume was increased to 1200 L, a volume at which approximately 35 mg L-1 h(-1) resveratrol per round was produced. This repeated fed-batch bioconversion process for resveratrol production is comparable to enzyme or cell immobilization strategies in terms of reusing cycles, but without incurring additional costs for immobilization. (C) 2017 Elsevier Ltd. All rights reserved.