Microalgae cells release O-2 during photosynthesis. The gas can accumulate and adhere in form of bubbles, which affect the transport of nutrients in the biofilm and the biofilm microstructure. To investigate the reasons for the adhesion of these oxygen bubbles and their impact on biofilm, polytetrafluoroethylene (PTFE) emulsion was sprayed onto glass surface to change the parameters for gas accumulation and adhesion. The results indicated gas could aggregate into bubbles and adhere to hydrophobic and rough surface. The bubble behaviors caused the biofilm to be porous (with a microporosity of 9.43-20.94%). The biomass concentration of the more porous biofilm increased by 9.26% to 22.42 g m(-2) on 1% PTFE-treated surface compared to that on an untreated surface. However, with an increase in PTFE concentration, the amount of adhered bubbles increased. More microalgae cells in biofilms were carried up by bubbles. The biofilm concentration on 5% PTFE-treated surface decreased by 15.30%.