A concentrated white wine vinasses stream (WV) was consecutively processed by a series of physical separation stages with two main targets; i) to produce a phenolic-rich product with antioxidant activity and ii) to reduce the initial pollutant load of WV. The optimal process could be defined based on a sequence of centrifugation and membrane filtration to selectively remove suspended and soluble solids and phenolic compounds. The final dried product contains 45% GAE (gallic acid equivalent) and presents an ABTS (2,20-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)) radical scavenging capacity equivalent to almost 2 g of Trolox. The total carbon, total phenolics and the chemical oxygen demand (COD) in the permeate stream from the nanofiltration stage were reduced by 85.8, 85.1 and 91.8%, respectively. The toxicity, evaluated using the Microtox method, was significantly reduced from the EC50 values of 10 (5)% in the original stream to 1-7%. (C) 2017 Elsevier B.V. All rights reserved.