Desulfurization of biogas is essential for its application in solid oxide fuel cells. The influence of CH4, CO2, H-2, and O-2 as well as the effect of moisture onto desulfurization performance of an activated carbon, an adsorbent based on a CuO-MnO mixture, and a zeolite adsorbent were analyzed. The use of moisturized gas had no negative influence on the H2S adsorption performance of activated carbon. The CuO-MnO sorbent showed the best performance, but the presence of moisture had a negative influence. The performance of zeolite dropped for three gas mixtures, while for two other mixtures moisture had little to no influence on H2S adsorption performance.