Nitrogen oxides in flue gas cause considerable environmental and health problems. In this study, the NO removal through Fe(II)EDTA absorption based anaerobic ammonium oxidation (Anammox) reduction was first evaluated in batch tests. The removal efficiency and removal rate of NO reached 74.5%-85.4% and 1.62-15.48 mu mol NO gVSS(-1) h(-1) with Fe(II)EDTA absorption, respectively. The optimal temperature and pH for Fe(II)EDTA absorption based Anammox reduction of NO was 30 degrees C and 7.0 in the range of temperature from 25 to 45 degrees C and pH from 6.5 to 8.0, respectively. The maximum specific substrate utilization rate and half-saturation constant of Fe(II)EDTA-NO were experimentally determined as 0.09 mmol N gVSS(-1) h(-1) and 1.338 mM, respectively. Then, a sequencing batch reactor (SBR1) performing Fe(II)EDTA absorption based Anammox reduction for NO removal was continuously, operated for 35 days. The NO removal performance of SBR1 decreased gradually, and the NO removal efficiency and NO removal rate decreased from 86.65% and 17.26 mu mol gVSS(-1) h(-1) to 43.64% and 12.75 mu mol gVSS(-1) h(-1), respectively. Meanwhile, the biomass, the diversity, and abundance of the bacterial community continually decreased in the sludge of SBR1, indicating that the reactor could not stably run when the Fe(II)EDTA-NO concentration was 0.09 mM. However, with improving Fe(II)EDTA-NO concentration and daily addition of a little biomass from an expanded granular sludge blanket reactor (EGSB) Anammox reactor, another sequencing batch reactor (SBR2) had stably run for 55 days. The average NO removal efficiency and NO removal rate reached 85.04% and 3.97 mM NO d(-1) (or 106.15 mu mol gVSS(-1) h(-1)), respectively. This is very interesting to ammonia based wet flue gas desulfurization, as the produced wastewater containing (NH4)(2)SO3 could be purified by Anammox to produce enough biomass.