Measuring natural ventilation rates in buildings with large vents with high accuracy and precision is not straight forward due to high spatial and temporal variabilities in the velocity distribution. Simplification of airflow rate measurements are mostly effectuated by lowering sampling density. Different sampling densities were investigated for both direct and tracer gas methods and compared with a detailed direct measurement method were in a naturally ventilated animal mock-up building. The results obtained by the reference method indicated that using only sampling locations in the middle of the side openings overestimated the airflow rate. In view of wind variations, better accuracy, precision and lower coefficients of variation were obtained with a higher number of sampling locations. The coefficients of variation varied between 5% for the reference and 29% using only one sampling location in the side outlet. In the ridge opening, only one middle sampling location was sufficient for an accuracy of 2% and a precision of 3%. The indirect tracer gas method gave varying concentrations with high confidence intervals resulting in non-significantly different measurement results between the different sampling strategies. The pattern of sampling locations was found to be very important resulting in different accuracies for a given sampling density. (C) 2017 Elsevier B.V. All rights reserved.