Bio-oil derived from the fast pyrolysis of lignin contains a range of chemicals including a lot of oxygenated chemical components which should be selectively upgraded to improve the oil quality. In this study, in-situ catalytic upgrading of bio-oil during the fast pyrolysis of lignin over five types of high aluminum zeolites, i.e., H-Ferrierite, H-Mordenite, H-ZSM-5, H-Beta and H-USY zeolites, were performed. It is found that the channel structure, pore sizes and acidity of zeolite had great effect on the product distribution, coke formation, and deoxygenation. The highest yield of light oil was obtained by using H-ZSM-5 zeolite and the highest selectivity towards monoaromatic hydrocarbons was achieved by H-Beta zeolite. This study could provide a guidance for the selection of suitable zeolite for the in-situ catalytic deoxygenation of bio-oil derived from fast pyrolysis of lignin.