Bubble size, which controls flotation kinetics, is strongly dependent on frother concentration. Frother concentration variations occur in operations due to the complexity of some circuits and the demands to deliver a reagent at part-per-million levels. A colorimetric technique for analysis of samples from industrial streams has been developed that enables frother deportment around flotation circuits to be determined. This exercise, referred to as frother distribution mapping, establishes whether frother delivery strategies reach the target concentrations and identifies where corrective action may be beneficial. A case study, which illustrates sampling and analytical protocols, reveals significant concentration differences between grinding circuit products, which were not eliminated by merging the streams. Other findings were concentration variations that suggested cycling of frother delivery, the presence of remnant frother in recycled process waters, and the occurrence of frother partitioning between pulp and froth. The experiences, particularly adaptations to local conditions, may help others in planning frother mapping exercises.