This study describes the formulation of new buccoadhesive films containing Myrrh extract as a natural and safe antimicrobial agent to give a long local medication. Buccoadhesive films of Myrrh extract were developed using solvent evaporation technique. The fabricated Myrrh extract films were evaluated for their palatability, weight, surface pH, and their content uniformity. In addition, tensile strength and the percentage of elongation of the films as well as in vitro mucoadhesive time were determined. Also, the films were tested for their antimicrobial activities. Obtained results showed that the tested films are elegant in appearance and have neutral pH, palatable by volunteers and have good tensile strength and elasticity. They also exhibited antibacterial activities against different types of bacterial strains as well as antifungal activity against Candida albicans.