In the present study, we coated cotton fabrics with protein-based nanoparticles containing vitamin E (atocopherol) by the pad-cure method. Scarming electron microscopy, Fourier transform infrared spectroscopy, and air permeability analysis of coated samples confirmed the fixation of the nanoparticles onto the fabric's surface. The antioxidant activity of the coated fabrics was evaluated by 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radicals reduction. Samples coated with nanoparticles containing the highest amount of encapsulated vitamin E (20% of the oil phase) showed the highest antioxidant activity. The protein-based coating was maintained for at least 10 washing cycles, demonstrating the reliability of the pad-cure method for the fixation of nanoparticles onto cotton surfaces. A methodology for nanoparticles release from the coated surfaces and their transfer to other substrates was demonstrated by the simple crock meter rubbing in the presence of sweat and protease. A high amount of material can be transferred and released to other substrates, such as textiles and skin, through the synergistic effect of sweat/protease and abrasion. An array of cosmetic and medical applications are possible with the developed coating and release methodology in which vitamin E would impart vital benefits as skin protection, anti-ageing product, or skin moisturizer.