화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Journal of Industrial and Engineering Chemistry, Vol.56, 62-73, December, 2017
DOI10.1016/j.jiec.2017.06.008  Export Citation
Theranostic carbon dots ‘clathrate-like’ nanostructures for targeted photo-chemotherapy and bioimaging of cancer
Sunil Pandey1, 2, Ganga Raju Gedda3, Mukeshchand Thakur1, Mukesh Lavkush Bhaisare1, Abou Talib4, M. Shahnawaz Khan4, Shou-Mei Wu2, and Hui-Fen Wu1, 2, †
  • 1Department of Chemistry and Center for Nanoscience and Nanotechnology, National Sun Yat-Sen University, 70, Lien-Hai Road, Kaohsiung 80424, Taiwan
  • 2School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan
  • 3Institute of Medical Science and Technology, National Sun Yat-Sen University, Kaohsiung 80424, Taiwan
  • 4Doctoral Degree Program in Marine Biotechnology, National Sun Yat-Sen University and Academia Sinica, Kaohsiung 80424, Taiwan
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The drug delivery application of carbon-based quantum dots is a new platform in the medical field. We report a unique morphological structure called carbon-dots clathrates (C-dotsCL) conjugated with folic acid (FA) for targeted delivery of methotrexate (MTX), an anti-cancer drug under physiological conditions followed by photothermal (PTT) and photodynamic (PDT) treatment on cancer cells. Under physiological milieu, the C-dotsCL-MTX-FA complex are pH-dependent and it can control release the anti-cancer drug in vitro. Furthermore, in vivo studies were also performed by using genetically-induced pancreatic cancer model to comprehend the in vivo pharmacokinetics and tissue distribution pattern of C-dotsCL-MTX-FA complex. The results demonstrated that the CQD was able to deliver high concentration of MTX in tumour tissues in contrast to the non-tumour tissues. The impact of near-infrared (NIR) laser (1064 nm; Nd-YAG) on the CQDs system was found to be extremely effective for the rapid release of MTX in tumour tissues. The generation of reactive oxygen species (ROS) by C-dotsCL upon irradiation of NIR laser was evaluated using flow cytometry. After conjugation with the C-dotsCL, the half-life of the MTX drug (t1/2), elimination constant (Kel), area under the curve (AUC) was drastically improved to prove the efficacy of C-dotCL as a powerful drug vehicle. This study has significantly improved the cancer theranostics research using C-dotsCL to act as a multi-functional nanomaterial for imaging and as a drug delivery agent.
Keywords:Carbon dots clathrates;Methotrexate;Photothermal;Reactive oxygen species;Elimination constant;Area under curve
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