화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Journal of Industrial and Engineering Chemistry, Vol.57, 339-348, January, 2018
DOI10.1016/j.jiec.2017.08.041  Export Citation
Antioxidant and antimicrobial activity of oils obtained from a mixture of citrus by-products using a modified supercritical carbon dioxide
John Ndayishimiye, Deok Jum Lim1, and Byung Soo Chun†
  • Department of Food Science and Technology, Pukyong National University, 45 Yongso-ro, Namgu, Busan 48513, Republic of Korea
  • 1Y.G. Company, 365, Shinsolo, Namgu, Busan 608-739, Republic of Korea
E-mail:
This study investigated the impact of combining citrus seeds and citrus peels on the bioactivity of the resulting oil obtained using a modified supercritical carbon dioxide. The total phenolic and total flavonoid contents were determined, and the citrus-peel and citrus-seed oils exhibited high content. In addition, antioxidant activity was determined, and the oil extracted using SC-CO2 + ethanol at 200 bar exhibited high IC50 values of 0.52 and 0.53 mg/ml for citrus-peel alone and mixture oils, respectively, for the DPPH assay. Oil from the mixture exhibited high antimicrobial activity, and the oils were more susceptible for gram-positive than gram-negative bacteria.
Keywords:Antioxidant activity;Antimicrobial activity;Mixture;Supercritical CO2 extraction;Modifier
  1. Mehl F, Marti G, Boccard J, Debrus B, Merle P, Delort E, Baroux L, Raymo V, Velazco MI, Sommer H, Food Chem., 143, 325 (2014)
  2. Laufenberg G, Kunz B, Nystroem M, Bioresour. Technol., 87(2), 167 (2003)
  3. Fisher K, Phillips C, Trends Food Sci. Technol., 19(3), 156 (2008)
  4. Matthaus B, Ozcan M, Grasas Aceites, 63(3), 313 (2012)
  5. Hayat K, Zhang XM, Chen HQ, Xia SQ, Jia CS, Zhong F, Sep. Purif. Technol., 73(3), 371 (2010)
  6. Manthey JA, Grohmann K, J. Agric. Food Chem., 49(7), 3268 (2001)
  7. Lee YH, Charles AL, Kung HF, Ho CT, Huang TC, Ind. Crop. Prod., 31(1), 59 (2010)
  8. Espina L, Somolinos M, Loran S, Conchello P, Garcia D, Pagan R, Food Chem., 22(6), 896 (2011)
  9. Calvo M, Angulo E, Costa-Batllori P, Shiva C, Adelantado C, Vicente A, Biotechnology, 5(2), 137 (2006)
  10. Lee OH, Lee BY, Bioresour. Technol., 101(10), 3751 (2010)
  11. Casquete R, Castro SM, Martin A, Ruiz-Moyano S, Saraiva JA, Cordoba MG, Teixeira P, Innov. Food Sci. Emerg. Technol., 31, 37 (2015)
  12. Anwar F, Naseer R, Bhanger M, Ashraf S, Talpur FN, Aladedunye FA, J. Am. Oil Chem. Soc., 85(4), 321 (2008)
  13. Adeyeye EI, Adesina AJ, Int. J. Curr. Microbiol. Appl. Sci., 4(5), 537 (2015)
  14. Raman G, Jayaprakasha GK, Cho MH, Brodbelt J, Patil BS, Sep. Purif. Technol., 45(2), 147 (2005)
  15. Illes V, Daood HG, Perneczki S, Szokonya L, Then M, J. Supercrit. Fluids, 17(2), 177 (2000)
  16. Yamini Y, Sefidkon F, Pourmortazavi S, Flavour Fragr. J., 17(5), 345 (2002)
  17. Pourmortazavi SM, Hajimirsadeghi SS, J. Chromatogr. A, 1163(1), 2 (2007)
  18. Salgin U, Doker O, Calimli A, J. Supercrit. Fluids, 38(3), 326 (2006)
  19. Diaz-Reinoso B, Moure A, Dominguez H, Parajo JC, J. Agric. Food Chem., 54(7), 2441 (2006)
  20. Shu XS, Gao ZH, Yang XL, Fitoterapia, 75, 656 (2004)
  21. Yu J, Dandekar DV, Toledo RT, Singh RK, Patil BS, Food Chem., 105(3), 1026 (2007)
  22. Ueno H, Tanaka M, Machmudah S, Sasaki M, Goto M, Food Bioprocess Technol., 1(4), 357 (2008)
  23. Aparicio R, Roda L, Albi MA, Gutierrez F, J. Agric. Food Chem., 47(10), 4150 (1999)
  24. Sun M, Temelli F, J. Supercrit. Fluids, 37(3), 397 (2006)
  25. Ndayishimiye J, Getachew AT, Chun BS, Waste Biomass Valorization (2016) 1, doi:http://dx.doi.org/10.1007/s12649-016-9697-8.
  26. Meda A, Lamien CE, Romito M, Millogo J, Nacoulma OG, Food Chem., 91(3), 571 (2005)
  27. Brahmi F, Mechri B, Flamini G, Dhibi M, Hammami M, Acta Physiol. Plant., 35(4), 1061 (2013)
  28. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C, Free Radic. Biol. Med., 26(9), 1231 (1999)
  29. Mann C, Markham J, J. Appl. Microbiol., 84(4), 538 (1998)
  30. De Castro MDL, Valcarcel M, Tena MT, Analytical Supercritical Fluid Extraction, Springer Science & Business Media, 2012.
  31. Lim GB, Lee SY, Lee EK, Haam SJ, Kim WS, Biochem. Eng. J., 11(2), 181 (2002)
  32. Ignat I, Volf I, Popa VI, Food Chem., 126(4), 1821 (2011)
  33. De Moraes Barros HR, De Castro Ferreira TAP, Genovese MI, Food Chem., 134(4), 1892 (2012)
  34. Gorinstein S, Zachwieja Z, Folta M, Barton H, Piotrowicz J, Zemser M, Weisz M, Trakhtenberg S, Martin-Belloso O, J. Agric. Food Chem., 49(2), 952 (2001)
  35. Pereira CG, Meireles MAA, Food Bioprocess Technol., 3(3), 340 (2010)
  36. Sanal IS, Bayraktar E, Mehmetoglu UU, Calimli A, J. Supercrit. Fluids, 34(3), 331 (2005)
  37. Choi ES, Noh MJ, Yoo KP, J. Chem. Eng. Data, 43(1), 6 (1998)
  38. Adil IH, Yener ME, Bayindirli A, Sep. Sci. Technol., 43(5), 1091 (2008)
  39. Adil IH, Cetin HI, Yener ME, Bayindirli A, J. Supercrit. Fluids, 43(1), 55 (2007)
  40. Cocero MJ, Calvo L, J. Am. Oil Chem. Soc., 73(11), 1573 (1996)
  41. Reverchon E, De Marco I, J. Supercrit. Fluids, 38(2), 146 (2006)
  42. Lou SN, Lai YC, Hsu YS, Ho CT, Food Chem., 197, 1 (2016)
  43. Zhang Y, Sun Y, Xi W, Shen Y, Qiao L, Zhong L, Ye X, Zhou Z, Food Chem., 145, 674 (2014)
  44. Karimi E, Oskoueian E, Hendra R, Oskoueian A, Jaafar HZ, Molecules, 17(2), 1203 (2012)
  45. Chen X, Yuan K, Liu H, J. Food Agric. Environ., 8(2), 150 (2012)
  46. Ghasemi K, Ghasemi Y, Ebrahimzadeh MA, Pak. J. Pharm Sci., 22(3), 277 (2009)
  47. Goulas V, Manganaris G, Food Chem., 131(1), 39 (2012)
  48. Nyam KL, Tan CP, Lai OM, Long K, Man YBC, Food Bioprocess Technol., 4(8), 1432 (2011)
  49. Beveridge TH, Girard B, Kopp T, Drover JC, J. Agric. Food Chem., 53(5), 1799 (2005)
  50. Lazos E, Servos D, Grasas Aceites, 39(4-5), 232 (1988)
  51. Materska M, Perucka I, J. Agric. Food Chem., 53(5), 1750 (2005)
  52. Amarowicz R, Pegg R, Rahimi-Moghaddam P, Barl B, Weil J, Food Chem., 84(4), 551 (2004)
  53. Kamal-Eldin A, Appelqvist LA, Lipids, 31(7), 671 (1996)
  54. Rudzinska M, Korczak J, Gramza A, Wasowicz E, Dutta PC, J. AOAC Int., 87(2), 499 (2004)
  55. Pande G, Akoh CC, Food Chem., 120(4), 1067 (2010)
  56. Perez-Jimenez J, Arranz S, Tabernero M, Diaz-Rubio ME, Serrano J, Goni I, Saura-Calixto F, Food Res. Int., 41(3), 274 (2008)
  57. Llorach R, Tomas-Barberan FA, Ferreres F, J. Agric. Food Chem., 52(16), 5109 (2004)
  58. Chen HY, Lin YC, Hsieh CL, Food Chem., 104(4), 1418 (2007)
  59. Tuberoso CI, Kowalczyk A, Sarritzu E, Cabras P, Food Chem., 103(4), 1494 (2007)
  60. Burt S, Int. J. Food Microbiol., 94(3), 223 (2004)
  61. Oliveira AA, Salvador AA, Smania A, Smania EF, Maraschin M, Ferreira SR, J. Biotechnol., 164(3), 423 (2013)
  62. Bakkali F, Averbeck S, Averbeck D, Idaomar M, Food Chem. Toxicol., 46(2), 446 (2008)