Functional silica nanoparticles conjugated with beta-glucan to deliver anti-tuberculosis drug molecules

Jangsun Hwang; Jaewoo Son; Youngmin Seo; Yeonho Jo; Kyungwoo Lee; Dohyun Lee; Muhammad Saad Khan; Sachin Chavan; Chanhwi Park; Anand Sharma; Assaf A. Gilad; Jonghoon Choi

Journal of Industrial and Engineering Chemistry, Vol.58, 376-385, February, 2018

DOI10.1016/j.jiec.2017.09.051 Export Citation

Functional silica nanoparticles conjugated with beta-glucan to deliver anti-tuberculosis drug molecules

Jangsun Hwang¹, Jaewoo Son¹, Youngmin Seo¹, Yeonho Jo¹, Kyungwoo Lee¹, Dohyun Lee¹, Muhammad Saad Khan¹, Sachin Chavan¹, Chanhwi Park¹, Anand Sharma², Assaf A. Gilad^{3, 4, †}, and Jonghoon Choi^{1, †}

¹School of Integrative Engineering, Chung-Ang University, Seoul 06974, Republic of Korea
²Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Kamrup, Assam 781039, India
³Russell H. Morgan Department of Radiology and Radiological Health, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
⁴Cellular Imaging Section, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA

E-mail:,

Bacterial infections and resistance against antibiotics are on the rise despite new drug development. New developments in the field of nanomedicine are proving to be an alternative for traditional antibiotics. Silica nanoparticles (SiNPs) have a promising role in emerging nanomedicine because of their low cytotoxicity and efficient drug delivery potential. In current study, we developed and analyzed silica nanoparticles of ∼50 nm in size that are capable of encapsulating small organic molecules and drugs, such as fluorescein isothiocyanate (FITC), doxorubicin (DOX), 4′, 6-diamidino-2-phenylindole (DAPI) and/or isoniazid (INH). Our drug delivery contains the anti-tuberculosis drug, INH, which is encapsulated in beta (β)-glucan-conjugated SiNPs. We focused on synthesizing and encapsulating SiNPs that have amine functional groups as well as the ability to conjugate with β-glucan molecules, making the nanocomplex both a drug carrier and a stimulus for host immune systems.

Keywords:Silica nanoparticles;(β)-glucan;Macrophages;Drug delivery;Immune response

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[2] Pan LM, He QJ, Liu JN, Chen Y, Ma M, Zhang LL, Shi JL, J. Am. Chem. Soc., 134(13), 5722 (2012)

[3] Slowing II, Vivero-Escoto JL, Wu CW, Lin VSY, Adv. Drug Deliv. Rev., 60, 1278 (2008)

[4] He C, Hu Y, Yin L, Tang C, Yin C, Biomaterials, 31, 3657 (2010)

[5] Ahmed N, Michelin-Jamois M, Fessi H, Elaissari A, Soft Matter, 8, 2554 (2012)

[6] Sun T, Zhang YS, Pang B, Hyun DC, Yang M, Xia Y, Angew. Chem.-Int. Edit., 53, 12320 (2014)

[7] Cheng R, Meng F, Deng C, Klok HA, Zhong Z, Biomaterials, 34, 3647 (2013)

[8] Laurent S, Saei AA, Behzadi S, Panahifar A, Mahmoudi M, Expert Opin. Drug Deliv., 11, 1449 (2014)

[9] Ochs M, Muhlfeld C, Am. J. Physiol. Lung. Cell. Mol. Physiol., 305, L15 (2013)

[10] Yan E, Ding Y, Chen C, Li R, Hu Y, Jiang X, Chem. Commun., 2718 (2009).

[11] Choi J, Kim K, Kim T, Liu G, Bar-Shir A, Hyeon T, McMahon MT, Bulte JW, Fisher JP, Gilad AA, J. Control. Release, 156, 239 (2011)

[12] Gerion D, Pinaud F, Williams SC, Parak WJ, Zanchet D, Weiss S, Alivisatos AP, J. Phys. Chem. B, 105(37), 8861 (2001)

[13] Lu J, Liong M, Li Z, Zink JI, Tamanoi F, Small, 6, 1794 (2010)

[14] Hwang J, Lee E, Kim J, Seo Y, Lee KH, Hong JW, Gilad AA, Park H, Choi J, Colloids Surf. B: Biointerfaces, 142, 290 (2016)

[15] Napierska D, Thomassen LC, Rabolli V, Lison D, Gonzalez L, Kirsch-Volders M, Martens JA, Hoet PH, Small, 5, 846 (2009)

[16] He X, Hai L, Su J, Wang K, Wu X, Nanoscale, 3, 2936 (2011)

[17] Lee J, Lee Y, Youn JK, Na HB, Yu T, Kim H, Lee SM, Koo YM, Kwak JH, Park HG, Small, 4, 143 (2008)

[18] Goyal G, Hwang J, Aviral J, Seo Y, Jo Y, Son J, Choi J, J. Ind. Eng. Chem., 47, 179 (2017)

[19] Tsoni SV, Brown GD, Ann. N.Y. Acad. Sci., 1143, 45 (2008)

[20] Sakurai K, Uezu K, Numata M, Hasegawa T, Li C, Kaneko K, Shinkai S, Chem. Commun., 4383 (2005).

[21] Kim YR, Hwang J, Koh HJ, Jang K, Lee JD, Choi J, Yang CS, Biomaterials, 86, 1 (2016)

[22] Chan GCF, Chan WK, Sze DMY, J. Hematol. Oncol., 2, 25 (2009)

[23] Brown GD, Taylor PR, Reid DM, Willment JA, Williams DL, Martinez-Pomares L, Wong SY, Gordon S, J. Exp. Med., 196, 407 (2002)

[24] Mochizuki S, Sakurai K, J. Contam. Hydrol., 151, 155 (2011)

[25] Sato M, Sano H, Iwaki D, Kudo K, Konishi M, Takahashi H, Takahashi T, Imaizumi H, Asai Y, Kuroki Y, J. Immunol., 171, 417 (2003)

[26] Yoo H, Pak J, J. Nanopart. Res., 15, 1609 (2013)

[27] Durgun G, Ocakoglu K, Ozcelik S, J. Phys. Chem., 115, 16322 (2011)

[28] Yokoi T, Yoshitake H, Yamada T, Kubota Y, Tatsumi T, J. Mater. Chem., 16, 1125 (2006)

[29] He Q, Shi J, Zhu M, Chen Y, Chen F, Microporous Mesoporous Mater., 131, 314 (2010)

[30] Huang X, Li L, Liu T, Hao N, Liu H, Chen D, Tang F, ACS Nano, 5, 5390 (2011)

[31] Mendoza A, Torres-Hernandez JA, Ault JG, Pedersen-Lane JH, Gao D, Lawrence DA, Cell Stress Chaperones, 19, 777 (2014)

[32] Yang EJ, Choi IH, Immune Netw., 13, 94 (2013)

[33] Nakagawa Y, Ohno N, Murai T, J. Infect. Dis., 187, 710 (2003)

[34] Volman JJ, Ramakers JD, J. Plat, Physiol. Behav., 94, 276 (2008)

[35] Ishibashi KI, Miura NN, Adachi Y, Ohno N, Yadomae T, Biosci. Biotechnol. Biochem., 65, 1993 (2001)

[36] Yanaki T, Kojima T, Norisuye T, Polym. J., 13, 1135 (1981)

[37] Surenjav U, Zhang L, Xu X, Zhang X, Zeng F, Carbohydr. Polym., 63, 97 (2006)

[38] Kimura T, Koumoto K, Sakurai K, Shinkai S, Chem. Lett., 29(11), 1242 (2000)

[39] Lee N, Hyeon T, Chem. Soc. Rev., 41, 2575 (2012)