Bone related-bacterial diseases including wound infections and osteomyelitis (OM) still remain a serious problem. In this study, a hybrid co-electrospun membrane consisting of gelatin (GE) and Poly(d, l-lactideco- glycolide) (PLGA) fibrous sheets containing different concentrations (0, 0.1, 0.5, and 1 wt%) of silver sulfadiazine (AgSD) was designed to provide for improved antimicrobial effect and biocompatibility. Well-defined products were characterized by physicochemical analyses. For biological in vitro assessments, mouse osteoblastic MC3T3-E1 cells were cultured on the scaffolds. This test was done in order to assay for cytotoxicity by measuring cell proliferation. Antibacterial activity against gram-negative Pseudomonas aeruginosa (P.aeruginosa), gram-positive Staphylococcus aureus (S. aureus), and Methicillinresistant Staphylococcus aureus (MRSA) was also tested. These biological tests showed that GE/PLGA-AgSD scaffolds had good cell viability, as well as effective antimicrobial activity. These remarkable results suggest that GE/PLGA-AgSD scaffolds possess great potential for the treatment of OM and can find many uses in the field of bone tissue engineering. (C) 2017 Elsevier B.V. All rights reserved.