In present study, the efficiency of three oleaginous yeasts i.e., Yarrowia lipolytica, Rhodotorula glutinis and Cryptococcus curvatus were compared for their lipid assimilation capacities using three different FWleachates as a medium. The FW-leachates were collected from dry anaerobic digesters and diluted to achieve carbohydrate content of 25 g L-1 prior to yeast inoculations. Around 5% of yeast cultures were individually mixed in three different FW-leachate mediums and incubated under 30 degrees C and 150 rpm agitation for 6 days. The Y. lipolytica produced high biomass with lipid contents of 49.0 +/- 2% on dry weight basis. Whereas, the acetic acid concentration of > 6 g L-1 inhibited the growth of R. glutinis. The study observed that the selection of appropriate FW-leachate composition is highly important for biolipid accumulation by oleaginous yeasts. (C) 2017 Elsevier Ltd. All rights reserved.