Natural, synthetic or semisynthetic antibiotics are highly used to prevent or treat diseases in humans and animals, and to promote animal growth. This fact makes that antibiotics residues or their transformation products may be present in food or in the environment after human or animal excretion. For this reason, it is imperative to develop reliable and sensitive analytical methodologies for their analysis. The main aim of this work is to present and discuss the most recent applications of capillary electromigration methods for the analysis of antibiotics, including the developments and applications of their use as chiral selectors in CE. The literature published from June 2015 to June 2017 is included following the previous review by Dominguez-Vega etal. (Electrophoresis, 2016, 37, 189-211). Information about the use of different detection systems, off-line and on-line strategies to improve sensitivity, and microchip devices for the analysis of antibiotics is provided and properly discussed.