A comprehensive kinetic modeling of Fischer-Tropsch synthesis (FTS) over Co-Ni/HZSM-5 nano-structure catalyst was developed in a fixed bed. To evaluate the chemical and physical properties of prepared catalyst and support, characterization methods of XRD, BET, H-2 chemisorption, TPR and NH3-TPD were performed. The proposed kinetic model was developed based on the alkyl mechanism. The re-adsorption and hydrogenation on the secondary sites of catalyst was included in developing kinetic model. To estimate the kinetic parameters, a series of experiments were conducted under operating conditions (i.e., temperature: 473-513 K, pressure: 10-25 bar, H-2/CO ratio of 1-2.5 and gas hourly space velocity: 900-2900 1/h). The significance and rationality of proposed comprehensive kinetic model was examined using the correlation and statistical tests. The results showed that the experimental data were well predicted by means of proposed model. (C) 2017 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.