A novel bimediator compound has been synthesized by covalently linking two redox mediators and used for fabrication of bimediator modified electrode to perform electrocatalytic oxidation and reduction of substrates. Ferrocene monocarboxylic acid and thionin have been linked via amide bond using (N-3-Dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC center dot HCl) and N-Hydroxy benzotriazole (HOBt) as cross-linking agents. The electrochemical characterization and surface characterization have confirmed the presence of both redox mediators in the bimediator compound on the modified electrode surface which has facilitated electron transfer between analyte and the electrode. The ferrocene has mediated electrocatalytic oxidation of dopamine at positive potentials while thionin has mediated electrocatalytic reduction of hydrogen peroxide at negative potentials. Effective utilization of this bimediator modified electrode has been examined under static and dynamic conditions by cyclic voltammetry and chronoamperometric techniques. Ferrocene has been found to oxidise dopamine at a potential of 0.35 V in the concentration range of 0.569 mu M to 515 mu M with a detection limit of 0.190 mu m and sensitivity of 142 mu A mM(-1) cm(-2) whereas thionin has been found to reduce hydrogen peroxide at a potential of - 0.3 V in the concentration range of 0.569 mu M to 785 mu m with a detection limit of 0.190 M and sensitivity of 128 mu A mM(-1) cm(-2). This bimediator modified electrode has also been used for real sample analysis.