Black glasses are amorphous materials based on silicon oxycarbide. The use of precursors in the form of ladder-like silsesquioxanes enables the control of the amount of carbon ions in the glass network by adjusting ratios of T to D structural units in precursors. In this study, four different sols precursors of four different layers of black glasses on titanium substrates were prepared. The materials were analyzed with the use of various spectroscopic and microscopic methods. Formation of continuous and hermetic layers resistant to corrosion was proven. The black glasses layers were examined as materials for biomedical applications. Therefore, preliminary tests of their bioactivity and biocompatibility were performed. The best results were obtained for the material of lower contribution of carbon ions.