Spontaneous organizations of designed elements with explicit shape and symmetry are essential for developing useful structures and materials. We report the topologically directed assemblies of four categories (a total of 24) of sphere rod conjugates, composed of a sphere-like fullerene (C-60) derivative and a rod-like oligofluorene(s) (OF), both of which are promising organic semiconductor materials. Although the packing of either spheres or rods has been well-studied, conjugates having both shapes substantially enrich resultant assembled structures. Mandated by their shapes and topologies, directed assemblies of these conjugates result not only in diverse unconventional semiconducting supramolecular lattices with controlled domain sizes but also in tunable charge transport properties of the resulting structures. These results demonstrate the importance of persistent molecular topology on hierarchically assembled structures and their final properties.