Direct observation of multiple reactive sites in the zeolite HZSM-5, a member of the MFI family of zeolite structures, contradicts the traditional view of only one type of active protonic species in industrially important zeolites. In addition to the well-known Bronsted acid site proton, two other protonic species undergo room-temperature hydrogen deuterium exchange with an alkane hydrocarbon reagent, including one zeolite moiety characterized by a broad H-1 chemical shift at ca. 12-15 ppm that is reported here for the first time. Although the ca. 13 ppm chemical shift value is consistent with computational predictions from the literature for a surface-stabilized hydroxonium ion in a zeolite, data suggest that the signal does not arise from hydroxonium species but rather from hydroxyls on extra-lattice aluminol species proximate to Bronsted lattice sites, i.e., a small population of highly deshielded acid sites. Double-resonance experiments show that this species is proximate to Al atoms, similar to the Bronsted acid site proton. These sites can be removed by appropriate postsynthesis chemical treatment, yielding a catalyst with reduced activity for isotopic H/D exchange reactions. Additionally, other extra-lattice aluminum hydroxyl groups previously discussed in the literature but whose protons were considered unreactive are also shown for the first time to react with hydrocarbon probe molecules. Two-dimensional exchange NMR reveals direct proton exchange between the Bronsted site and these two types of extra-lattice Al OH species, and it also reveals unexpected proton exchange between extra-lattice Al OH species and an alkane reagent.