Synthesis and electropolymerization of a new conjugated dithieno(3,2-b: 2',3'-d) pyrole (DTP) derivative, namely, 2-(2-(2-(4H-dithieno[3,2-b: 2',3'-d] pyrrol-4-yl) ethoxy) ethoxy) ethanamine, (DTP-alkoxy-NH2) monomer are presented. The electrochemical, spectroelectrochemical effects and biosensor capability towards to glucose oxidase enzyme of P(DTP-alkoxy-NH2) polymer film are investigated in detail during the placement of alkoxy-NH2 functional unit in the polymer. Electrochemical and optical results between neutral and oxidized states of the polymer film show that (P(DTP-alkoxy-NH2)) has reversibly multi-electrochromic behaviors. Furthermore, glucose oxidase enzyme (GOx) is covalently immobilized on Au electrodes modified with P(DTP-alkoxy-NH2) conducting polymer. This enzyme is entrapped into conducting polymer during electropolymerization of DTP monomer on the electrodes. Some characterization parameters (Maximum reaction rate, Michaelis-Menten constants, temperature, pH and operational stabilities) for this kind of biosensor application is also determined. (C) 2017 Elsevier Ltd. All rights reserved.