The oceans have been shown as an almost entire reserve of novel enzymes. Alkaline phosphatase (AP) is a key enzyme in the marine environment. This review provides an account of the alkaline phosphatase production of marine organisms and is based on reports of different species of marine origin, from primitive bacteria to vertebrates such as fishes. Purification and enzymatic characterization, investigation of activators and inhibitors, use as biosensor based inhibition activities, ecological roles in sea, and eventually potential application of alkaline phosphatase from different species of marine organisms are overviewed. Recent biotechnological progresses require the researches of new enzymes from marine resources that can be used for various applications. Marine enzyme resources might be of great interest for biotechnological processes because of their safety accompanied with natural origin. The specific features presented by marine enzymes can be advantageously used both for process improvement or to develop new processes and products. This review highlights the potential of marine organism as valuable resources of AP. The overview of AP in different marine organisms showed its use in various fields among biotechnology being increased and can be promising candidate in future.