표면 개질된 금나노입자의 제조 및 이의 측방유동면역 센서 응용

김동석; 최봉길; Dong Seok Kim; Bong Gill Choi

Applied Chemistry for Engineering, Vol.29, No.1, 97-102, February, 2018

DOI10.14478/ace.2017.1109 Export Citation

표면 개질된 금나노입자의 제조 및 이의 측방유동면역 센서 응용

Preparation of Surface Functionalized Gold Nanoparticles and their Lateral Flow Immunoassay Applications

김동석, 최봉길 ^†

강원대학교 화학공학과

Dong Seok Kim, and Bong Gill Choi^†

Department of Chemical Engineering, Kangwon National University, 346 Joongang-ro, Samcheok, Gangwon-do 25913, South Korea

E-mail:

초록

본 연구에서는 높은 민감성을 가진 측방유동면역분석(lateral flow immunoassay) 스트립 센서를 제작하기 위하여 mercaptoundecanoic acid (MUA)와 L-lysine 단분자를 사용하여 금나노입자 표면을 개질할 수 있는 합성법을 개발하였다. 균일한 사이즈의 금나노입자를 합성하기 위하여 Turkevich-Frens 합성법을 이용하였으며 16.7 ± 2.1 nm 크기의 금나노 입자를 제조하였다. 기능화된 금나노입자의 특성을 확인하기 위하여 투과전자현미경(TEM), 자외선-가시광선 분광광도계(UV-vis spectroscopy), X선 광전자분광기(XPS), 푸리에 변환 적외선 분광기(FT-IR)를 사용하여 분석하였다. 금나노입자와 항체 간의 안정적인 접합(conjugation)을 위한 pH 및 항체의 농도 조건은 pH 7.07, 항원의 농도 10 μg/mL로 최적화되었다. B형간염 표면항원을 검출하기 위하여 측방유동면역분석 스트립 센서를 제작하였으며, 표면개질된 금나노 입자로 제작된 면역스트립 센서에서 10 ng/mL로 낮은 검출한계를 나타내었으며, 이는 기능화되지 않은 금나노입자 기반 면역스트립센서의 100 ng/mL보다 높았다.

In this work, the surface of gold nanoparticles (AuNPs) was modified with small molecules including mercaptoundecanoic acid (MUA) and L-lysine for the development of highly sensitive lateral flow (LF) sensors. Uniformly sized AuNps were synthesized by a modified Turkevich-Frens method, showing an average size of 16.7 ± 2.1 nm. Functionalized AuNPs were then characterized by transmission electron microscopy, UV-vis spectroscopy, X-ray photoelectron spectroscopy, and Fourier transform infrared spectroscopy. The stable conjugation of AuNPs and antibodies was obtained at pH 7.07 and the antibody concentration of 10 μg/mL. The functionalized AuNP-based LF sensor exhibited lower detection limit of 10 ng/mL for hepatitis B surface antigens than that of using the bare AuNP-based LF sensor (100 ng/mL).

Keywords:gold nanoparticle;functionalization;hepatitis B surface antigen;lateral flow immunoassay

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[3] Yetisen AK, Akram MS, Lowe CR, Lab Chip, 13, 2210 (2013)

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