Effect of solution viscosity on retardation of cell sedimentation in DLP 3D printing of gelatin methacrylate/silk fibroin bioink

Kyunga Na; Sungchul Shin; Hyunji Lee; Donghyeok Shin; Jihye Baek; Hojung Kwak; Minsung Park; Jonghyun Shin; Jinho Hyun

Journal of Industrial and Engineering Chemistry, Vol.61, 340-347, May, 2018

DOI10.1016/j.jiec.2017.12.032 Export Citation

Effect of solution viscosity on retardation of cell sedimentation in DLP 3D printing of gelatin methacrylate/silk fibroin bioink

Kyunga Na¹, Sungchul Shin², Hyunji Lee², Donghyeok Shin², Jihye Baek³, Hojung Kwak², Minsung Park², Jonghyun Shin², and Jinho Hyun^{1, 2, †}

¹Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea
²Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, Seoul 08826, Republic of Korea
³Kukdong R&D Center, 135 Hasinbeonyeong-ro, Saha-gu, Busan 49432, Republic of Korea

E-mail:

To improve the cell suspension of the gelatin-methacrylate(GelMA) bioink, the viscosity of the ink solution was controlled by biocompatible silk fibroin(SF) particles. The viscosity of the GelMA solution increased about 1.4 x 10 2 folds by the addition of 1 w/v% SF particles. The increase of solution viscosity reduced the displacement of cells and retarded the cell sedimentation significantly. SF-GelMA bioink containing 1 w/v% SF was non-cytotoxic and used for the 3D bioprinting by directly projecting a feature onto the bioink. The cells encapsulated in the structures showed a high level of metabolic activity and demonstrated that SF-GelMA was a biocompatible material.

Keywords:Digital light processing (DLP) 3D printing;Cell sedimentation;Solution viscosity;Silk fibroin particle;Gelatin-methacrylate (GelMA)

[References]

Murphy SV, Atala A, Nat. Biotechnol., 32, 773 (2014)
Jia W, Gungor-Ozkerim PS, Zhang YS, Yue K, Zhu K, Pi Q, Byambaa B, Dokmeci M, Shin SR, Khadhemhosseini A, Biomaterials, 106, 58 (2016)
Kim BS, Jang J, Char S, Gao G, Kong JS, Ahn M, Cho DW, Biofabrication, 8, 035013 (2016)
Tan XP, Tan YJ, Chow CSL, Tor SB, Yeong WY, Mater. Sci. Eng. C-Biomimetic Supramol. Syst., 76, 1328 (2017)
Castilho M, Rodrigues J, Pires I, Gouveia B, Pereira M, Moseke C, Groll J, Ewald A, Vorndran E, Biofabrication, 7, 015004 (2015)
Jang J, Park HJ, Kim SW, Kim H, Park JY, Na SJ, Kim HJ, Park MN, Choi SH, Park SH, Biomaterials, 112, 264 (2017)
Bertassoni LE, Cardosa JC, Manoharan V, Cristino AL, Bhise NS, Araujo WA, Zorlutuna P, Vrana NE, Ghaemmaghami AM, Dokmeci R, Biofabrication, 6, 024105 (2014)
Zhu W, Qu X, Zhu J, Ma X, Patel S, Liu J, Wang P, Lai CS, Gou M, Xu, Zhang YK, Chen S, Biomaterials, 124, 106 (2017)
Mironov V, Prestwich G, Forgacs G, J. Mater. Chem., 17, 2054 (2007)
Billiet T, Gevaert E, De Schryver T, Cornelissen M, Dubruel P, Biomaterials, 35, 49 (2014)
Yeo Y, Geng WL, Ito T, Kohane DS, Burdick JA, Radisic M, J. Biomed. Mater. Res. B, 81B, 312 (2007)
Ruoslahti E, Annu. Rev. Cell Dev. Biol., 12, 697 (1996)
Nichol JW, Koshy ST, Bae H, Hwang CM, Yamanlar S, Khademhosseini A, Biomaterials, 31, 5536 (2010)
Lawrence BD, Cronin-Golomb M, Georgakoudi I, Kaplan DL, Omenetto FG, Biomacromolecules, 9(4), 1214 (2008)
Li M, Tao W, Kuga S, Nishiyama Y, Polym. Adv. Technol., 14, 694 (2003)
Shirahama H, Lee BH, Tan LP, Cho NJ, Sci. Rep., 6, 31036 (2016)
Ki CS, Kim JW, Oh HJ, Lee KH, Park YH, Int. J. Biol. Macromol., 41, 346 (2007)
Morcol T, Subramanian A, Velander WH, J. Immunol. Methods, 203, 45 (1997)
Hoch E, Hirth T, Tovar GE, Borchers K, J. Mater. Chem. B, 1, 5675 (2013)
Xiaoqin W, Kluge JA, Leisk GG, Kaplan DL, Biomaterials, 29, 1054 (2008)
Jin HJ, Kaplan DL, Nature, 424, 1057 (2003)
Einstein A, Ann. Phys., 17, 549 (1905)
Shaw DJ, Introduction to Colloid and Surface Chemistry, fourth ed., Butterworth-Heinemann, London, 1992, pp. 21.
Milo R, Phillips R, Cell Biology by the Numbers, Garland Science, New York, 2015 pp. 72.
Yue K, Santiago G, Alarez M, Tamayol A, annabi N, Khademhosseini A, Biomaterials, 73, 254 (2015)
Fang Q, Chen D, Yang Z, Li M, Mater. Sci. Eng. C-Biomimetic Supramol. Syst., 29, 1527 (2009)

[Referenced By]

[1] Murphy SV, Atala A, Nat. Biotechnol., 32, 773 (2014)

[2] Jia W, Gungor-Ozkerim PS, Zhang YS, Yue K, Zhu K, Pi Q, Byambaa B, Dokmeci M, Shin SR, Khadhemhosseini A, Biomaterials, 106, 58 (2016)

[3] Kim BS, Jang J, Char S, Gao G, Kong JS, Ahn M, Cho DW, Biofabrication, 8, 035013 (2016)

[4] Tan XP, Tan YJ, Chow CSL, Tor SB, Yeong WY, Mater. Sci. Eng. C-Biomimetic Supramol. Syst., 76, 1328 (2017)

[5] Castilho M, Rodrigues J, Pires I, Gouveia B, Pereira M, Moseke C, Groll J, Ewald A, Vorndran E, Biofabrication, 7, 015004 (2015)

[6] Jang J, Park HJ, Kim SW, Kim H, Park JY, Na SJ, Kim HJ, Park MN, Choi SH, Park SH, Biomaterials, 112, 264 (2017)

[7] Bertassoni LE, Cardosa JC, Manoharan V, Cristino AL, Bhise NS, Araujo WA, Zorlutuna P, Vrana NE, Ghaemmaghami AM, Dokmeci R, Biofabrication, 6, 024105 (2014)

[8] Zhu W, Qu X, Zhu J, Ma X, Patel S, Liu J, Wang P, Lai CS, Gou M, Xu, Zhang YK, Chen S, Biomaterials, 124, 106 (2017)

[9] Mironov V, Prestwich G, Forgacs G, J. Mater. Chem., 17, 2054 (2007)

[10] Billiet T, Gevaert E, De Schryver T, Cornelissen M, Dubruel P, Biomaterials, 35, 49 (2014)

[11] Yeo Y, Geng WL, Ito T, Kohane DS, Burdick JA, Radisic M, J. Biomed. Mater. Res. B, 81B, 312 (2007)

[12] Ruoslahti E, Annu. Rev. Cell Dev. Biol., 12, 697 (1996)

[13] Nichol JW, Koshy ST, Bae H, Hwang CM, Yamanlar S, Khademhosseini A, Biomaterials, 31, 5536 (2010)

[14] Lawrence BD, Cronin-Golomb M, Georgakoudi I, Kaplan DL, Omenetto FG, Biomacromolecules, 9(4), 1214 (2008)

[15] Li M, Tao W, Kuga S, Nishiyama Y, Polym. Adv. Technol., 14, 694 (2003)

[16] Shirahama H, Lee BH, Tan LP, Cho NJ, Sci. Rep., 6, 31036 (2016)

[17] Ki CS, Kim JW, Oh HJ, Lee KH, Park YH, Int. J. Biol. Macromol., 41, 346 (2007)

[18] Morcol T, Subramanian A, Velander WH, J. Immunol. Methods, 203, 45 (1997)

[19] Hoch E, Hirth T, Tovar GE, Borchers K, J. Mater. Chem. B, 1, 5675 (2013)

[20] Xiaoqin W, Kluge JA, Leisk GG, Kaplan DL, Biomaterials, 29, 1054 (2008)

[21] Jin HJ, Kaplan DL, Nature, 424, 1057 (2003)

[22] Einstein A, Ann. Phys., 17, 549 (1905)

[23] Shaw DJ, Introduction to Colloid and Surface Chemistry, fourth ed., Butterworth-Heinemann, London, 1992, pp. 21.

[24] Milo R, Phillips R, Cell Biology by the Numbers, Garland Science, New York, 2015 pp. 72.

[25] Yue K, Santiago G, Alarez M, Tamayol A, annabi N, Khademhosseini A, Biomaterials, 73, 254 (2015)

[26] Fang Q, Chen D, Yang Z, Li M, Mater. Sci. Eng. C-Biomimetic Supramol. Syst., 29, 1527 (2009)