The effects of pH, transmembrane pressure, and ZnCl2 concentration on the purification of glucose from solution through nanofiltration were investigated. The solutions used contained ZnCl2 and glucose in concentrations as high as 180 and 80 g/L, respectively. A high concentration of glucose was discovered in the retentate as the product, and ZnCl2 was filtered out into the permeate; the highest separation factor achieved was 10. The derivative Hagen Poiseuille equation was applied to model the experimental data and calculate the effective thickness and pore radius of the membranes used. Moreover, an integrated multiple-nanofiltration system was designed to process a real bagasse hydrolysis solution. The fermentability of the purified sugar solution was confirmed, with an 82% total alcohol yield obtained. The multiple-nanofiltration system is both economically and environmentally sustainable for the production of bioethanol through chemical hydrolysis of biomass.