This study investigated the possibility of enhancing bioethanol production by combined pretreatment methods for water hyacinth. Three different kinds of pretreatment methods, including microbial pretreatment, microbial combined dilute acid pretreatment, and microbial combined dilute alkaline pretreatment, were investigated for water hyacinth degradation. The results showed that microbial combined dilute acid pretreatment is the most effective method, resulting in the highest cellulose content (39.4 +/- 2.8%) and reducing sugars production (430.66 mg.g(-1)). Scanning Electron Microscopy and Fourier Transform Infrared Spectrometer analysis indicated that the basic tissue of water hyacinth was significantly destroyed. Compared to the other previously reported pretreatment methods for water hyacinth, which did not append additional cellulase and microbes for hydrolysis process, the microbial combined dilute acid pretreatment of our research could achieve the highest reducing sugars. Moreover, the production of bioethanol could achieve 1.40 g.L-1 after fermentation, which could provide an extremely promising way for utilization of water hyacinth.