The present study demonstrates utilization of secondary agricultural wastes for xylitol production. The highest xylan-to-xylose (70%) conversion was achieved using dilute nitric acid as catalyst followed by resin treatment. Results show that resin treatment efficiently removed nitrate salt (70%), phenolic content and 5-HMF (70%). Highest xylitol yield (85%) was achieved during fermentation using Candida tropicalis MTCC 6192 from the neutralized hemicellulosic hydrolysate medium. Good recovery (> 15%) was achieved from corncob with 85% xylose to xylitol conversion during fermentation. This two-step process for transformation of agri-waste to xylitol is much simpler and it could possibly be considered for up scaling after process optimization parameters.