Our investigations seek to illustrate the use of fluorescence spectral deconvolution to characterize multiple partitioning sites for aromatic chromophores within aqueous and reverse micelles. The spatial distribution of solutes within micellar systems is dictated by a variety of noncovalent interactions. To probe far multiple partitioning microenvironments, we use the aromatic fluorophore Prodan with its extensive solubility in a range of media and its appreciable spectral sensitivity to the polarity of its surroundings. We conduct a systematic study of the partitioning of Prodan in aqueous micellar systems with anionic, cationic, zwitterionic, and nonionic surfactant headgraups and in reverse micellar systems with both anionic and cationic surfactant headgroups. By deconvoluting the overall Prodan fluorescence emission spectrum into a sum of overlapping Gaussian functions, the principal microenvironments of the Prodan molecules may be ascertained. Fluorescence data are consistent with the location of Prodan in a variety of sites, including partitionings that may be influenced by electrostatic, hydrophobic, dipolar, and cation-pi interactions in both aqueous and reverse micelles.