A newly fabricated nitrogen-doped graphene (NGE) supported hemin composite was prepared with facile one-pot method deriving from graphene oxide. Hemin was directly anchored on the surface of NGE during the N-doping process with doping-reducing agent of 2-aminopyridine. XPS results revealed the nitrogen doping of graphene and the integration of Hemin with NGE, and the hemin-N-C (H-NGE) composite was characterized with TEM and SEM. The peroxidase-like activity of hemin maintained, displaying fast redox property. The H-NGE also exhibited remarkable electrocatalysis towards O-2 and H2O2 reduction due to the high-speed charge-transfer between hemin and the modified electrode. The good electroactivity of NGE and the excellent synergic effect enables an enhanced electrochemical performance. Based on the well-designed H-NGE, the electrocatalytic H2O2 determinations was monitored amperometrically with a low detection limit (0.092 mu M). (c) 2017 Elsevier Ltd. All rights reserved.