In the support of developing a substitute for diesel fuel automobiles, the research in renewable energy has been focused due to the hurly-burly situation for petroleum combat and environmental causes. The present study has been carried out in a naturally aspirated light-duty variable compression ratio (VCR) multi-fuel research engine. As input amends, three significant input parameters as injection pressure (IP), compression ratio (CR) and injection timing (IT) have been taken. In this test, the input parameters are taken as 210 bar, 230 bar, 250 bar for IP and 16, 17, 18 for CR and 21 degrees, 23 degrees, 25 degrees before top dead center (bTDC) for IT. To outline the resulting output parameters like performance and emissions, the statistical tool like the design of experiments (DoE) have been used for planning the experimental trials. The lesser exhaust pollution and better performance are the desirable output factors by optimizing the input parameters via factorial design. For validating the models developed using response surface methodology (RSM), the confirmatory tests have been carried out to portray the combined effects of CR, IP and IT on the engine characteristics using all test fuels. Maximum Brake thermal efficiency of 30.05% was found for F(1) fuel at 230 bar IP and 18 CR with 23 degrees bTDC IT. Minimum carbon monoxide of 0.41% was observed at IP of 230 bar and CR of 18 with 25 degrees bTDC IT and oxides of nitrogen of 205.7 ppm was found at 250 bar IP and 16 CR with 25 degrees bTDC IT for F(1) fuel.