Amide linkage containing two nicotinic acid amphiphiles have been investigated for their gel-emulsion and glucose sensing properties. In emulsions, the synthesized materials were used as stabilizers in water medium, whereas organic solvents and/or mineral oils as dispersed phase and water acts as a continuous phase. The gel-emulsions were prepared at room temperature by stirring or shaking, and no heating-cooling arrangement or addition of any other co-solvents or active agents are mandatory. Rheological investigation of these amphiphiles showed mechanical stability of gel-emulsions and its viscoelasticity. Optical images confirmed the existence of network structures in gel-emulsion phase for both amphiphiles. FE-SEM measurement suggests the morphology depends on the solvent:water composition. An XRD study proposed that the arrangements of the amphiphiles in the emulsion state are different. The gel-emulsions formed by the studied amphiphiles are able to entrap and release biomolecules, anticancer drug molecules, and hazards chemicals. These gelator molecules are very sensitive to detect glucose molecules in aqueous solution. The subchronic toxicity of these synthesized molecules has been evaluated as nontoxic on the hematological and biochemical parameters of male Swiss albino mice.