The corrosion inhibitor of nitrogen-containing amino acid histidine (His) has been applied in brown oxidation solutions and the relationships between brown oxidation solutions with the various concentrations of His and the adhesion strength of copper/resin laminates have been systematically studied by various testing techniques, including electrochemical impedance spectroscopy (EIS), peeling strength and field emission scanning electronic microscope. The result obtained from EIS suggested that His exhibited excellent anti-corrosive performance which contributed to a higher stability of the organic metallic film post brown oxidation process treatment. The roughness surface like honeycomb structure was gained and peel strength of the Cu/resin laminates was raised up to 0.71 kg/cm by altering the concentration of His in brown oxidation solutions. Moreover, theoretical calculations manifested that the studied inhibitor was almost adsorbed in parallel on the copper surface and X-ray photoelectron spectroscopy (XPS) declared that the value of adhesion strength was related to the surface chemistry of copper foil and resins after the lamination process which may attribute to chemical reactions at the copper/resin interface.