Complicated purification steps, together with the fact that beta-glucosidase has to be tolerant to ethanol restricts the application of beta-glucosidase in isoflavone aglycone hydrolyzing process. beta-Glucosidase Bgl1A(A24S/F297Y) is a promising enzyme in hydrolyzing isoflavones. In this work, six different carbohydrate-binding modules (CBMs), which were from 3 families, were fused to the C-terminal of Bg11A(A24S/F297Y), respectively, to simplify the enzyme preparation process. The fusion proteins were expressed in Escherichia coli and adsorbed onto cellulose. The Bgl-CBM24 was found to have the highest immobilization efficiency at room temperature within 1 h adsorption. Notably, 1-g cellulose absorbs up to 254.9 +/- 5.7 U of Bgl-CBM24. Interestingly, the immobilized Bgl-CBM24 showed improved ethanol tolerance ability, with the IC50 of 35% (v/v) ethanol. Bgl-CBM24 effectively hydrolyze soybean isoflavone glycosides. The hydrolysis rate of daidzin and gemistin was 85.22 +/- 3.24% and 82.14 +/- 3.82% within 10 min, with the concentrations of daidzein and genistein increased by 6.36 +/- 0.18 mM and 3.98 +/- 0.22 mM, respectively. In the repetitive hydrolytic cycles, the concentrations of daidzein and genistein still increased by 3.07 +/- 0.24 mM and 1.94 +/- 0.34 mM in the fourth cycle with 20% (v/v) ethanol. These results suggest that the immobilized Bgl-CBM24 has excellent potential in the preparation of isoflavone aglycones. (C) 2017, The Society for Biotechnology, Japan. All rights reserved.