Here we tested the bioconversion of biodiesel-derived crude glycerol by the oleaginous red yeast Sporidiobolus pararoseus ICM281507 in two bioreactors types (stirred-tank and airlift). High production yields (biomass, 10.62 +/- 0.21 g/L; lipids, 3.26 +/- 0.13 g/L; (beta-carotene, 30.64 +/- 0.05 mg/L; total carotenoids, 46.59 +/- 0.07 mg/L) were achieved in a 3.0 L airlift bioreactor under uncontrolled pH regimes (initial pH 5.63). Under optimized conditions (6.0 vvm aeration rate; 60 +/- 5% constant dissolved oxygen [DO] maintained by flushing pure oxygen [O-2] into the vessel; 10,000 Lux light irradiation) volumetric production in the airlift bioreactor was further increased (biomass, 19.30 +/- 1.07 g/L; lipids, 6.61 +/- 0.04 g/L, beta-carotene, 109.75 +/- 0.21 mg/L; total carotenoids 151.00 +/- 2.71 mg/L). Production was also recorded at a S. pararoseus KIVI281507 growth rate of 0.16 +/- 0.00 h(-1) (lipids, 0.94 +/- 0.04 g/L/d; beta-carotene, 15.68 +/- 0.40 mg/L/d; total carotenoids, 21.56 +/- 0.20 mg/L/d). Lipids from S. pararoseus ICM281507 had a high unsaturated fatty acid content, with oleic acid (C18:1) accounting for 80% of all fatty acids. This high oleic acid content makes S. pararoseus KM281507 well-suited as a third generation biodiesel feedstock. Our findings show that airlift bioreactors are suitable for bioconversion of crude glycerol into lipids and carotenoids using S. pararoseus ICM281507. This approach is advantageous because of its ease of operation, cost efficiency, and low energy consumption. (C) 2017, The Society for Biotechnology, Japan. All rights reserved.