The kinetics of negatively charged silver nanoparticle (AgNP) deposition on the supporting fibrinogen monolayers of well-characterized coverage was determined by the atomic force microscopy (AFM). The kinetics was quantitatively interpreted in terms of the hybrid random sequential adsorption model. The electrokinetic properties of the fibrinogen monolayers and fibrinogen/AgNP bilayers were thoroughly characterized in situ by the streaming potential measurements. These results were interpreted in terms of the general electrokinetic model expressing the particle coverage in terms of the zeta potential of the bilayers. This allowed one to determine the adsorption constants and the binding energy of AgNPs, which was equal to -20.8 and -21.3 kT for pH 3.5 and 7.4, respectively. These results confirmed the end-on mechanism of fibrinogen adsorption and the presence of positively charged spots at its molecule at pH 7.4 where it exhibits an average negative charge. Besides significance to basic science, the obtained results can be exploited for developing a procedure for producing AgNP monolayers of well-defined coverage and controlled particle release profile. (C) 2017 Elsevier Inc. All rights reserved.