Hydrogels are promising 3D materials that have demonstrated increasing applications in the encapsulation and delivery of drugs and cells. Herein we report an injectable colloidal hydrogel that directly assembled by graphene oxide (GO) and thermo-sensitive nanogels (tNG). The pH dependent hydrogen bonding interactions between the carboxyl and oxethyl groups induce the reversible assembly of GO and nano gels. The hydrogel is mouldable and can be shaped into different macroscopic objects, and the mechanical strengths are tunable with pH and temperature adjustment. The hybrid hydrogel by its own possesses high antibacterial activity, and demonstrates responsive drug release behaviour and high viability of 3D encapsulated cells. We expect this hybrid colloidal hydrogel can serve as an interesting scaffold for active cargo delivery and cell culture. (C) 2017 Published by Elsevier Inc.