The present work involves the synthesis of porous gelatin/AcA (PGE-AcA) hydrogel and novel porous gelatin-silver/AcA (NPGESNC-AcA) nanocomposite hydrogel, and their ability as effective biosorbents for the removal of Cu2+ ions from contaminated water. The formation of the samples was confirmed by UV-Vis, TEM, SEM, EDX, DLS, AFM, XRD, TGA/DTG and FTIR techniques. The adsorption studies results showed that maximum mono layer adsorption capacity of copper ions for PGE-AcA was achieved about 130.50 mg g(-1) in pH 6.0 for 50 min, and adsorption capacity for the NPGESNC-AcA was nearly 147.10 mg g(-1) in pH 5.5 for 40 min by atomic absorption spectroscopy technique. The Cu2+ ions loaded on the PGE-AcA and NPGESNC-AcA could be recovered by HCl above 65.8% and 78.7% after five consecutive cycles of adsorption/desorption, respectively. The results showed that the both of biosorbents loaded by Cu2+ ions could be easily regenerated and reusable. On the other hand, the results of adsorption kinetics and equilibrium isotherms were indicated high correlation coefficient (closer to a unit) for the pseudo-second-order and excellent fitted the adsorption data with the Langmuir isotherm model. Furthermore, the antimicrobial efficiency of the synthesized samples were tested on the Staphylococcus aureus and the Escherichia coil.