Langmuir monolayers were used to study the influence of four commercially applied parabens on multi component systems composed of lipid species characteristic of the cellular membrane of microorganisms found in carbohydrates and proteins reaching products, including food and cosmetics. The aim of the undertaken studies was to shed new light on the problem of parabens' interactions with membrane lipids and their affinity for monolayers differing with regard to the composition, mutual lipid ratios, and physicochemical properties. The discussion is based on the pi-A isotherm characteristics, surface morphology observation performed with BAM, and analysis of the diffraction data collected for the periodically ordered lipid domains present in the investigated multicomponent films. Our studies revealed that the selected parabens are capable of surface film modification and that the magnitude of this effect increases with the number of methylene groups in the ester part of paraben molecules. We found that the strongest destructive effect was observed for model 1 (Staphylococcus aureus), a lower effect was observed for model 2 (Pseudomonas aeruginosa), and the lowest effect was observed for model 3 (Candida albicans). It was inferred that such a trend appears due to the composition of the artificial membranes, i.e., above all, in the presence or lack of sterol molecules and the content of negatively charged lipids.