In this study, the potential use of palm pressed-fibers oil was studied as a potential source of biodiesel. The palm pressed-fibers oil was extracted by using supercritical fluid carbon dioxide at the isotherms 40 and 60 degrees C and at pressures 150-450 bar. The oil was transesterified under the following conditions: ethanol:oil molar ratio of 20:1 and 40:1; temperatures ranging from 150 to 350 degrees C; pressure of 200 bar, and a CO2 mass of 5.31 x 10(-7) g for 10-60 min. The palm pressed-fibers presented phenolic and flavonoid content of 4.37 +/- 0.19 mgGAE/g and 0.27 +/- 0.05 mgRE/g, respectively. The highest yield of palm pressed-fibers oil was 6.09% dry basis obtained at 40 degrees C and 450 bar. The palm pressed-fibers oils had a high level of saturation (more 80%) and low level of carotenoids (5.32-26.11 mu g/g oil). The transesterification conditions that generated the highest yield (molar ratio of 40:1, 150 degrees C for 60 min) produced oil consisting of 99.33% ethyl esters.