Composite scaffolds were prepared from the mixture of chitosan (C), gelatin (G) and nanodiopside (nDP) in different inorganic/organic weight ratios using the freeze-drying method. The prepared nDP and composite scaffolds were investigated using BET, FT-IR, SEM and XRD techniques. The composite scaffolds had 70-81% porosities with interlinked porous networks. Moreover, investigation of the cell proliferation, adhesion and viability using MTT test and mouse preosteoblast cell proved the cytocompatible nature of the composite scaffolds with improved cell attachment and proliferation. All these results essentially illustrated that this composite could have a potential ability for the tissue engineering applications.