Here we describe few novel grading agents (GrA) for the production of graded agaroses directly from seaweed aqueous extract under ambient conditions. Graded agaroses exhibits gelling properties in a wider range (gel strength >= 100->= 1600 gcm(-2), gelling temperature <= 27->= 35 degrees C, and melting temperature <= 72->= 90 degrees C) which are essential for a variety of molecular biology applications. The uniqueness of this process is the ability to tailor the essential properties of agarose for desired applications, can be achieved from single seaweed aqueous extract by altering initiator nature and/or concentration (w/w) of an initiator in GrA.